CiBER-Seq

CRISPR interference with barcode expression reporter sequencing

CiBER-Seq is a technique for measuring quantitative profiles of the changes in a specific molecular phenotype, such as gene expression, across a comprehensive library of CRISPR-mediated genetic perturbations. These measurements are made by bulk sequencing of RNA and DNA extracted from pooled populations, and don’t require fluorescent protein expression or cell sorting. CiBER-Seq directly profiles RNA abundance, which is often advantageous relative to protein-based reporters. However, it can be adapted to study protein-level phenotypes, such as regulated translation or degradation, by using a heterologous transcription factor to transduce that regulation into RNA expression.

This site is a resource for CiBER-Seq, based on our work in Muller et al., Science 2020. It builds on our genome-wide CRISPRi library described in McGlincy et al., BMC Genomics 2021