This site documents how to operate and construct the Ingolia lab turbidostat.
Operating the turbidostat. Prior to starting a continuous culture, a growth chamber and a media reservoir are assembled and sterilized. The sterile reservoir is then filled with media. These fluidics are then assembled into the turbidostat controller: the growth chamber goes into a turbidity detector and the media line is inserted into a peristaltic pump. At this point, the controller can be used to fill the growth chamber with media, and it can be inoculated to start the culture.
Constructing the turbidostat. Building the turbidostat requires assembling a custom PCB and mounting it in a 3D printed housing. The turbidostat also requires fluidics that are constructed using readily accessible plastic fittings and silicone tubing.